RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi

Abstract Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with wide market share. Due to intrinsic advantages, liquid fermentation Fusarium fujikuroi has become sole method for industrial GA3 production, but broader application hindered by low titer. In this study, we combined atmospheric room-temperature plasma (ARTP) ketoconazole-based screening obtain mutant strain 3-6-1 high yield GA3. Subsequently, medium composition parameters were systematically optimized increase titer GA3, resulting 2.5-fold compared obtained under initial conditions. Finally, considering prone substrate inhibition glucose repression, new strategy fed-batch was adopted 575.13 mg/L, which 13.86% higher than control. The random mutagenesis selection optimization developed study provides basis subsequent research on Graphical